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Medical Biotechnology Laboratory,
Faculty of Medicine,
University of Malaya,
50603 Kuala Lumpur,
MALAYSIA
Tel : 03-7967 7535
Fax : 03-7967 7536
Email: selvee@um.edu.my
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| CENTRAL RESEARCH EQUIPMENT |
| Charged services are offered for sample analysis and use of the following equipment: |
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| 1. MALDI TOF/TOF (ABI 4800 Plus) and TEMPO MALDI-LC |
| 2. PROTEIN CHIP SELDI SYSTEM (PBS II, Biorad) |
| 3. BIACORE 3000 (GE Healthcare) |
| 4. HIGH CONTENT SCREENING (Cellomics, Inc./VTi Array Scan) |
| 5. XCELLIGENCE REAL TIME CELL ANALYZER (Roche) |
| 6. HIGH GAS CHROMATOGRAPHY MASS SPECTROMETER (GCMS –QP2010, Shimadzu) |
| 7. LC-MS/MS (API 5500 QTRAP, Shimadzu) |
| 8. LC-MS/MS (API 3200, Shimadzu) |
| 9. MICROARRAY (Agilent Technologies) |
| 10. FLOWCYTOMETRY SYSTEM (FACsCANTO II) |
| 11. HISTOLOGY LABORATORY |
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For more information on cost and terms of use of equipment, please contact the person in charge in respective centres.
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 MALDI TOF/TOF (ABI 4800 Plus) and TEMPO MALDI-LC
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 Location : Proteomics Facility , Medical Biotechnology Laboratory
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Description :
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 BIACORE 3000 (GE Healthcare) |
 Location : UM Centre for Proteomics Research ( UMCPR) , Medical Biotechnology Laboratory |
| Description : |
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The BIAcore 3000 system is an instrument that uses SPR (Surface Plasmon Resonance) technology for measuring the interaction of macromolecules with each other, and with small ligands. One of the ligands is immobilized on carboxymethylated dextran over a gold surface, while the second partner (analyte) is captured as it flows over the immobilized ligand surface. Interaction is detected via SPR, in real time, at high sensitivity, without the use of radioactivity.
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| BIACORE 3000 System |
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Typical applications and uses:
The BIAcore can be used for measuring binding constants of macromolecular interactions (protein-protein, nucleic acids-proteins, protein ligand ), epitope mapping, ranking antibody affinities, concentration measurements, characterization of mutant proteins and ligand fishing.
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Contact Person:
Ms. Jaime Jacqueline, email : umcpr@um.edu.my
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| HIGH CONTENT SCREENING (Cellomics, Inc./VTi Array Scan) |
| Location : Centre for Cell Biology and Drug Discovery, Department of Pharmacology. |
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ArrayScan VTI HCS Reader is a modular High Content Screening instrument designed for high-capacity automated fluorescence imaging and quantitative analysis of fixed and live cells. The instrument can be utilised for rapid and high content cellular screening of the bioactivity and toxicology of drugs and natural products including traditional herbal medicines, as well as analysis of chemical and biological compound.
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Contact :
For enquiries or further information, please contact:
Dr. Cheah Shiau Chuen, cheahsc@um.edu.my, Office No: 03 - 7967 5720
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| HIGH GAS CHROMATOGRAPHY MASS SPECTROMETER (GCMS –QP2010, Shimadzu) |
| Location : Shimadzu - Centre for Xenobiotics Studies (SUCXES), Department of Pharmacology. |
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The GCMS –QP2010 Series Instrument is a bench top type gas chromatograph/ mass spectrometer intended for high-precision GC/MS analysis. The instrument enables mass spectrum measurement for qualitative analysis or identification of unknowns and Selected Ion Monitoring (SIM) measurement for quantitation of trace constituents.
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Contact :
For enquiries or further information, please contact:
Emmy Dayana Ahmad, emmy_117@hotmail.com: Office No : 03 - 7967 5722 / 5726
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| LC-MS/MS (API 3200, Shimadzu) |
| Location : Shimadzu - Centre for Xenobiotics Studies (SUCXES), Department of Pharmacology. |
| Description : |
| The API 3200 LC/MS/MS system is a fully-integrated triple quadrupole mass spectrometer built using premier technology that combines sensitivity and selectivity with ruggedness and reliability, which incorporate an Atmospheric Pressure Ionization (API) Ion source. |
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Features:
Mass Accuracy (with internal reference): 20 mmu
Dynamic Range: 1 cps to 4x106 cps (pulse counting)
Mass Range: m/z 5-1,700-quad mode and 50-1,700- linear ion trap mode
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Scan speed: up to 2400 amu/sec in quad mode, up to 4000 amu/sec in linear ion trap mode
Contact:
For enquiries or further information on the LC-MS/MS systems, please contact:
Didi Erwandi Mohammad Haron, didisucxes@gmail.com, Office No : 03 - 7967 5722 / 5726 |
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FLOWCYTOMETRY SYSTEM (FACsCANTO II)
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| Location : National Orthopaedic Centre of Excellence in Research and Learning (NOCERAL ) |
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The FACsCANTO II system is a technique for counting and examining microscopic particles suspended in a stream of fluid. It allows simultaneous multiparametric analysis of the physical and chemical characteristics of single cells flowing through an optical and electronic detection appratus. Certain applications may include physical sorting of components.
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Contact:
For enquiries or further information, please contact:
Dr. Sangeetha Naveen (TEG), Office No: 03 - 7967 7543, sangeevv@gmail.com
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| DOWNLOAD FORMS |
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FLOWCYTOMETRY REQUEST FORM |
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FLOWCYTOMETRY BOOKING FORM |
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HISTOLOGY LABORATORY
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Location :Genomic Facility, Medical Biotechnology Laboratory
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Description :
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Histology Laboratory offers histology services to both internal and external clients. The services include tissue preparation, embedding, sectioning, slide mounting, routine & special staining. The Histology Laboratory provides two types of services: a full end-to-end service provided by trained staff, or a “Do it Yourself” service providing access to laboratory facilities, specialist equipment and consumables.
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Access to the histological equipment is available to postgraduate students and staff at moderate rates. Users must undergo training according to the Standard Operating Procedures. Equipment available includes:
- Paraffin wax processor
- Paraffin wax embedder
- Rotary Microtome
- H & E staining set up
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The Histology Laboratory operates during working hour from 9.00am-5.00pm every day, except public holidays and weekends.
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Histology Laboratory aims to provide teaching, clinical and research tools to study the internal structure of biological tissues and cells using light microscope. Application of histology involves identifying and characterising diseases, evaluating tissue regeneration, analysing cellular tissue and localising protein structures (antigens).
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Following are the instrument in Histology Lab :
1. Thermo Scientific Microm STP 120 Spin Tissue Processor
2. Thermo Scientific Microm EC 350 Modular tissue embedding center
3. Thermo Scientific Microm HM 340 E Rotary Microtome
4. GFL Tissue Float Water Baths - 1052
5. Medax Specimen Stage-Stretching Table
6. Sastec Ice Flake Machine
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Contact Person:
Dr. M.R. Murali (TEG), , Office No: 03 - 7967 7543, mrmurali08@gmail.com
Dr. Sangeetha Naveen (TEG), Office No: 03 - 7967 7543, sangeevv@gmail.com
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| DOWNLOAD FORMS |
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Histology Request Form |
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Histology Booking Form |
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