Translational Core Laboratory (TCL)

MALDI TOF/TOF (ABI 4800 Plus) and TEMPO MALDI-LC

Location: Proteomics Facility , Medical Biotechnology Laboratory

Description:

Typical applications and uses:

  • Proteins MW measurements ranging 10 kDa and up to 300 kDa.
  • Protein identification by Peptide Mass Fingerprint (PMF) and tandem MS analysis.
  • De novo sequencing.
  • In conjunction with the adequate sample preparation pipeline: peptide and protein profiling.
  • Mass spectrometric read-out for kinetic enzymatic studies.
  • Post translational modifications (PTM) characterization.
  • Stable isotope based relative quantitation.

Contact Person:

Ms. Norsihah (MBL), Office No : 03 – 7967 7535, proteomics@um.edu.my
Ms. Jaime Jacqueline (UMCPR), umcpr@um.edu.my

DOWNLOAD FORMS
1. MALDI Request / Booking Form Click to download

PROTEIN CHIP SELDI SYSTEM (PBS II, Biorad)

Location: UM Centre for Proteomics Research ( UMCPR) , Medical Biotechnology Laboratory

Description:

The system uses surface-enhanced laser desorption/ionization (SELDI) technology which combines two powerful techniques: chromatography and mass spectrometry. The system integrates ProteinChip arrays, a SELDI reader and SELDI software to analyze samples based on measured time-of-flight (TOF).

Typical applications and uses:
The system will be mainly used in protein profiling and biomarker discovery.

BIACORE 3000 (GE Healthcare)

Location: UM Centre for Proteomics Research ( UMCPR) , Medical Biotechnology Laboratory

Description: The BIAcore 3000 system is an instrument that uses SPR (Surface Plasmon Resonance) technology for measuring the interaction of macromolecules with each other, and with small ligands. One of the ligands is immobilized on carboxymethylated dextran over a gold surface, while the second partner (analyte) is captured as it flows over the immobilized ligand surface. Interaction is detected via SPR, in real time, at high sensitivity, without the use of radioactivity.

Typical applications and uses:
The BIAcore can be used for measuring binding constants of macromolecular interactions (protein-protein, nucleic acids-proteins, protein ligand ), epitope mapping, ranking antibody affinities, concentration measurements, characterization of mutant proteins and ligand fishing.

Contact Person:

Ms. Jaime Jacqueline,
email : umcpr@um.edu.my

HIGH CONTENT SCREENING (Cellomics, Inc./VTi Array Scan)

Location: Centre for Cell Biology and Drug Discovery, Department of Pharmacology.

Description: ArrayScan VTI HCS Reader is a modular High Content Screening instrument designed for high-capacity automated fluorescence imaging and quantitative analysis of fixed and live cells. The instrument can be utilised for rapid and high content cellular screening of the bioactivity and toxicology of drugs and natural products including traditional herbal medicines, as well as analysis of chemical and biological compound.

Contact :

For enquiries or further information, please contact:
Dr. Looi Chung Yeng,
looicy@um.edu.my,
Office No: 03 – 7967 5720

XCELLIGENCE REAL TIME CELL ANALYZER (Roche)

Location: Centre for Cell Biology and Drug Discovery, Department of Pharmacology.
SELDI-ToF Proteinchip System

Description: Roche´s xCELLigence analyzer is an electrical impedance screening system for label free, real time monitoring of cellular events, providing a quantitative, kinetics data of the dinamic cellular events.

Contact :

For enquiries or further information, please contact:
Dr. Looi Chung Yeng,
looicy@um.edu.my,
Office No : 03 – 7967 5720

DOWNLOAD FORMS
1. EIS Request Form Click to download
2. EIS Booking Form Click to download
3. HCS Request Form Click to download
4. HCS Booking Form Click to download

HIGH GAS CHROMATOGRAPHY MASS SPECTROMETER (GCMS –QP2010, Shimadzu)

Location: Shimadzu – Centre for Xenobiotics Studies (SUCXES), Department of Pharmacology.

Description: The GCMS –QP2010 Series Instrument is a bench top type gas chromatograph/ mass spectrometer intended for high-precision GC/MS analysis. The instrument enables mass spectrum measurement for qualitative analysis or identification of unknowns and Selected Ion Monitoring (SIM) measurement for quantitation of trace constituents.

 Contact :

For enquiries or further information, please contact:
Emmy Dayana Ahmad,
emmy_117@hotmail.com:
Office No: 03 – 7967 5722 / 5726

LC-MS/MS (API 5500 QTRAP, Shimadzu)

Location: Shimadzu – Centre for Xenobiotics Studies (SUCXES), Department of Pharmacology.

Description:

Liquid chromatography-mass spectrometry is an analytical chemistry technique that combines the physical separation capabilities of liquid chromatography with the mass analysis capabilities of mass spectrometry. LC-MS is a powerful technique used for many applications which has very high sensitivity and selectivity.

Generally its application is oriented towards the specific detection and potential identification of chemicals in the presence of other chemicals (in a complex mixture).

The QTRAP MS is a hybrid Triple Quadrupole/Ion Trap mass spectrometer that can be used in several modes depending on the application. QTRAP method provided for a scan time of only 2.8 s to produce both MRM and and enhanced product ion scan.

LC-MS/MS (API 3200, Shimadzu)

Location: Shimadzu – Centre for Xenobiotics Studies (SUCXES), Department of Pharmacology.

Description: The API 3200 LC/MS/MS system is a fully-integrated triple quadrupole mass spectrometer built using premier technology that combines sensitivity and selectivity with ruggedness and reliability, which incorporate an Atmospheric Pressure Ionization (API) Ion source.

Features:

Mass Accuracy (with internal reference): 20 mmu
Dynamic Range: 1 cps to 4×106 cps (pulse counting)
Mass Range: m/z 5-1,700-quad mode and 50-1,700- linear ion trap mode

Scan speed: up to 2400 amu/sec in quad mode, up to 4000 amu/sec in linear ion trap mode

Contact:

For enquiries or further information on the LC-MS/MS systems, please contact:
Didi Erwandi Mohammad Haron,
didisucxes@gmail.com,
Office No : 03 – 7967 5722 / 5726

MICROARRAY (Agilent Technologies)

Location: Tropical Infectious Diseases Research and Education Centre (TIDREC)

Description: Microarray System consists of bead array reader and bead scan data acquisition for genetic analysis using a wide range of bead chips that house whole human/mouse genome or specific group of genes.

Contact:

For enquiries or further information, please contact:
Ms Juraina Jamil (TIDREC),
email: juraina@ummc.edu.my,
Office No : 03 – 7967 6670

FLOWCYTOMETRY SYSTEM (FACsCANTO II)

Location: National Orthopaedic Centre of Excellence in Research and Learning (NOCERAL )

Description:

The FACsCANTO II system is a technique for counting and examining microscopic particles suspended in a stream of fluid. It allows simultaneous multiparametric analysis of the physical and chemical characteristics of single cells flowing through an optical and electronic detection appratus. Certain applications may include physical sorting of components.

Contact:

For enquiries or further information, please contact:
Dr. Sangeetha Naveen (TEG),
Office No: 03 – 7967 7543,
sangeevv@gmail.com

DOWNLOAD FORMS
1. FLOWCYTOMETRY REQUEST FORM Click to download
2. FLOWCYTOMETRY BOOKING FORM Click to download

HISTOLOGY LABORATORY

Location: Genomic Facility, Medical Biotechnology Laboratory

Description: Histology Laboratory offers histology services to both internal and external clients. The services include tissue preparation, embedding, sectioning, slide mounting, routine & special staining. The Histology Laboratory provides two types of services: a full end-to-end service provided by trained staff, or a “Do it Yourself” service providing access to laboratory facilities, specialist equipment and consumables.

Access to the histological equipment is available to postgraduate students and staff at moderate rates. Users must undergo training according to the Standard Operating Procedures. Equipment available includes:

  • Paraffin wax processor
  • Paraffin wax embedder
  • Rotary Microtome
  • H & E staining set up

The Histology Laboratory operates during working hour from 9.00am-5.00pm every day, except public holidays and weekends.

Typical applications and uses:
Histology Laboratory aims to provide teaching, clinical and research tools to study the internal structure of biological tissues and cells using light microscope. Application of histology involves identifying and characterising diseases, evaluating tissue regeneration, analysing cellular tissue and localising protein structures (antigens).

Following are the instrument in Histology Lab :

1. Thermo Scientific Microm STP 120 Spin Tissue Processor
2. Thermo Scientific Microm EC 350 Modular tissue embedding center
3. Thermo Scientific Microm HM 340 E Rotary Microtome
4. GFL Tissue Float Water Baths – 1052
5. Medax Specimen Stage-Stretching Table
6. Sastec Ice Flake Machine

Contact Person:

Dr. M.R. Murali (TEG),
Office No: 03 – 7967 7543,
mrmurali08@gmail.com

Dr. Sangeetha Naveen (TEG),
Office No: 03 – 7967 7543,
sangeevv@gmail.com

DOWNLOAD FORMS
1. Histology Request Form Click to download
2. Histology Booking Form Click to download