Research

Research

Research Interest

The Department of Parasitology, places utmost importance in research on parasitic diseases and emerging parasites. The department has acquired many grants and translated its activities in the form of publications and presentations at national and international conferences.

Studies on pathogenicity and immunological characterization of Acanthamoeba species isolated from clinical and environtmental samples are currently being carried out. Development of recombinant antigens for the diagnosis of toxoplasmosis continues to be a major focus. Many molecular markers have been successfully identified and the search for a better diagnostic method is being developed. The department continues to perform sero-prevalence studies of Toxoplasmosis in both immunocompetent and immunocompromised patients to determine base-line data and infection rates. Emerging parasitic infections have prompted the Department to develop molecular technology for Cryptosporidiosis.

The department also gives emphasis on studies concerning detection of Cryptosporidium andGiardia in recreational and sewage water. Studies on Cryptosporidium and Giardia in HIV patients are also being carried out. The department is also looking into the viability and genotyping of Cryptosporidium. Studies on the biology, immunology and pathology ofBlastocystis hominis continues to be one of the core research activities. The reactivity ofBlastocystis isolates with monoclonals using immunoperoxidase technique is currently being evaluated. We are also trying to establish the detection protocol for Blastocystis cysts from water samples. The emphasis has also been in comprehending the biology and pathogenicity of Trichomonas vaginalis and microsporidium. The search for newer drugs against parasitic infections continues with emphasis on screening for herbal extracts from Malaysian traditional medicinal plants against malaria. The understanding of the mechanism and mode of action at molecular levels of these drugs against the target organisms provide the basis for the search of newer allopathic and herbal extracts against these organisms. Work is also being carried out on gene expression analysis of erythrocyte infected withPlasmodium sp. Work on molecular genetics and detection of Brugia pahangi andPlasmodium knowlesi (zoonotic simian malaria parasite) are currently being carried out. There are also efforts to look into the In vitro and in vivo studies of Plasmodium knowlesi. On the frontiers of entomological research, study on Aedes mosquitoes has become the thrust activity in the department. Research in entomotoxicology of forensically important insects and study on diversity of forensically important fly species are being carried out. These activities and available expertise have established the Department to be a national referral center for emerging and remerging parasitic infections.

On Going Research

  1. Prof. Dr. Johari Surin. Application of Mitochondrial DNA Analysis in the Identification of Cadaveric Dipteran Larvae for Postmortem Interval Estimation (PMI).
  2. Prof. Dr. Johari Surin. Dynamic Trends of the Phylogenetic Taxonomy of water borne protozoan parasites of man, animal agents and the environment in Malaysia based on Ssu Rrna and Gp60 Gene.
  3. Prof. Dr. Johari Surin. Phylogenetic Study of Cryptosporidium Isolated from Human, Animal and Aquatic Environment in Malaysia based on SSU rRNA and GP60 Genes.
  4. Prof. Dr. Johari Surin and Assoc. Prof. Dr Yvonne Lim Ai Lian. Genotyping ofCryptosporidium in Immunocompromised Patients.
  5. Prof. Dr. Johari Surin and Nor Adzliyana Binti Yaacob. Entomotoxicology of Forensically Important Insects and Its Effects on Larval Growth, Rate of Development and the Post Mortem Interval Estimation.
  6. Prof. Dr. Johari Surin and Asma Iqbal. Detection and Molecular Characterization of Cryptosporidium in HIV patients.
  7. Prof. Dr. Rohela Mahmud. Molecular Epidemiology of Microsporidia Isolated from Immunocompromised Patients.
  8. Prof. Dr. Rohela Mahmud and Azdayanti binti Muslim. Filariasis in Sub-Urban Areas of Klang Valley : Parasitological, Entomological and Molecular Studies.
  9. Prof. Dr. Jamaiah Ibrahim. Morphological Characterization oh Human Parasites Using Electron Microscopy.
  10. Prof. Dr. Suresh Kumar. Studies of the Correlation Between Biochemical and Molecular Aspects of Blastocystis with Fundamental Immunopathological Mechanisms During In vitro and In vivo.
  11. Prof. Dr. Suresh Kumar. Molecular and Biochemical Characterization of Isolates and Life Cycle Stages of Blastocystis hominis Isolated from Patients Suffering from Irritable Bowel Syndrome.
  12. Prof. Dr. Suresh Kumar and Lee II Li. Establishing the Detection Protocol forBlastocystis Cysts from Water Samples.
  13. Assoc. Prof. Dr. Zurainee Mohamed Nor. Study on the Anti-Malarial Activities of Malaysian Traditional Medicinal Plants.
  14. Assoc. Prof. Dr. Zurainee Mohamed Nor and Abdulelah Hussein Ahmad Aladhroey. In vivo Anti-Malarial Study on Malaysian Medicinal Plants. PS
  15. Assoc. Prof. Dr. Fong Mun Yik. Molecular Studies of Zoonotic Simian Malaria Parasite Plasmodium knowlesi.
  16. Assoc. Prof. Dr. Fong Mun Yik. Determination of Factors that Contribute to Protection Relationship of Pre-Existing Neutralizing Antibody Level.
  17. Assoc. Prof. Dr. Fong Mun Yik and Asha a/p Thanabalan. Molecular Genetic of the Filarial Worm Brugia pahangi.
  18. Assoc. Prof. Dr. Fong Mun Yik and Marshita binti Mohd Idris. Expression ofToxoplasma gondii Dense Granule Antigen (GRA2) in the Yeast Pichia pastoris for use in serodiagnosis of Toxoplasmosis.
  19. Assoc. Prof. Dr. Fong Mun Yik and AbdulSalam Mohammed Al-Mekhlafi. Molecular Epidemiology of Malaria Parasite in Yemen. PS175/2008C (RM28,500.00).
  20. Assoc. Prof. Dr Veeranoot Nissapatorn. Detection of Toxoplasma Responses in Saliva and Urine Samples of Patients with Psychological Disorders.
  21. Assoc. Prof. Dr Yvonne Lim Ai Lian. Helminth Infection and Asthma in the Orang Asli Communities.
  22. Assoc. Prof. Dr Yvonne Lim Ai Lian and Romano Ngui. Epidemiological Distribution of Soil-Transmitted Helminthes Infection and Its Association with Asthma among the Orang Asli Communities in Malaysia.
  23. Dr. Tan Tian Chye. Establishment of Continuous In vitro Culture of the Malaria Parasite Plasmodium knowlesi Obtained from Human.
  24. Dr. Noraishah Mydin Hj. Abdul Aziz. Does Closure of the Spinal Neural Tube Occur Asymmetrically?
  25. Dr. Noraishah Mydin Hj. Abdul Aziz. The Role of T-Cadherin During Adhesion and Fusion of the Spinal Neural Tube.
  26. Dr. Lau Yee Ling. Recombinant expression of Toxoplasma gondii Surface Antigens SAG1-SAG2.
  27. Dr. Lau Yee Ling. Development of DNA and Recombinant Vaccines againstToxoplasma gondii infection.